A simple, sensitive, reproducible, and cost-effective UV-spectrophotometric method has been developed and validated for the estimation of terbinafine hydrochloride in semisolid dosage forms. The method was based on detection of terbinafine hydrochloride at 221 nm using phosphate buffer solution pH 5.8 as the solvent system since this allows detection of the drug at the pH of skin (5-6). The developed method was validated with respect to linearity, accuracy (recovery), precision (inter-day and intra-day variations) and sensitivity (LOD and LOQ). Linearity was observed in the concentration range of 1-5 μg/ml with correlation coefficient of 0.9938. Results of the analysis were validated statistically and by recovery study. The per cent recovery of terbinafine hydrochloride ranged from 98.47–102.03%. The developed method was found to be precise with % RSD value of 0.903%. The study concluded that the UV-spectrophotometric method could be used for the quantification of terbinafine hydrochloride in pure form as well as in pharmaceutical formulations.
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